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| 10.05.2025 | 16:00 | Sigma Olomouc B | - | FK MAS Táborsko | 0:3 (0:1) | |
| 11.05.2025 | 10:15 | Slavia Praha B | - | Zbrojovka Brno | 1:1 (0:0) | |
| 10:15 | Viktoria Žižkov | - | AC Sparta Praha B | 0:1 (0:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | FK Varnsdorf | 1:1 (1:0) | ||
| 17:00 | Vysočina Jihlava | - | MFK Chrudim | 1:1 (0:0) | ||
| 17:00 | FC Zlín | - | MFK Vyškov | 2:1 (2:0) | ||
| 18:00 | 1. SK Prostějov | - | Sk Lisen | 1:0 (0:0) | ||
| 12.05.2025 | 17:00 | Baník Ostrava B | - | SFC Opava | 0:0 (0:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
FC Zlín | 28 | 20 | 7 | 1 | 43:13 | 30 | 67 |
| 2 |
|
MFK Chrudim | 28 | 15 | 7 | 6 | 46:27 | 19 | 52 |
| 3 |
|
MFK Vyškov | 28 | 10 | 10 | 8 | 29:24 | 5 | 40 |
| 4 |
|
FK MAS Táborsko | 28 | 10 | 8 | 10 | 33:29 | 4 | 38 |
| 5 |
|
AC Sparta Praha B | 28 | 9 | 10 | 9 | 40:38 | 2 | 37 |
| 6 |
|
FC Sellier & Bellot Vlašim | 28 | 8 | 13 | 7 | 41:38 | 3 | 37 |
| 7 |
|
1. SK Prostějov | 28 | 9 | 10 | 9 | 31:39 | -8 | 37 |
| 8 |
|
Sk Lisen | 28 | 8 | 12 | 8 | 27:31 | -4 | 36 |
| 9 |
|
Viktoria Žižkov | 28 | 10 | 6 | 12 | 45:45 | 0 | 36 |
| 10 |
|
SFC Opava | 28 | 9 | 8 | 11 | 29:37 | -8 | 35 |
| 11 |
|
Zbrojovka Brno | 28 | 8 | 11 | 9 | 37:40 | -3 | 35 |
| 12 |
|
Vysočina Jihlava | 28 | 8 | 11 | 9 | 34:38 | -4 | 35 |
| 13 |
|
Slavia Praha B | 28 | 8 | 10 | 10 | 40:34 | 6 | 34 |
| 14 |
|
Baník Ostrava B | 28 | 8 | 7 | 13 | 34:45 | -11 | 31 |
| 15 |
|
FK Varnsdorf | 28 | 7 | 8 | 13 | 34:41 | -7 | 29 |
| 16 |
|
Sigma Olomouc B | 28 | 5 | 6 | 17 | 26:50 | -24 | 21 |
| Aufstieg | |
| Relegation | |
| Abstieg |
