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| 23.04.2022 | 10:15 | MFK Chrudim | - | Viktoria Žižkov | 0:0 (0:0) | |
| 10:15 | FK Dukla Praha | - | FK Varnsdorf | 0:1 (0:0) | ||
| 10:15 | FK Fotbal Třinec | - | AC Sparta Praha B | 2:2 (2:2) | ||
| 10:15 | MFK Vyškov | - | Zbrojovka Brno | 0:2 (0:0) | ||
| 16:30 | Vysočina Jihlava | - | 1. SK Prostějov | 1:0 (0:0) | ||
| 17:00 | SK Líšeň | - | FK MAS Táborsko | 1:1 (0:0) | ||
| 17:00 | SFC Opava | - | 1. FK Příbram | 1:1 (0:1) | ||
| 17:00 | FC Sellier & Bellot Vlašim | - | FK Ústí nad Labem | 3:0 (2:0) |
| # | Mannschaft | Sp. | S. | U. | N. | Tore | Dif. | Pk. | |
|---|---|---|---|---|---|---|---|---|---|
| 1 |
|
Zbrojovka Brno | 25 | 19 | 2 | 4 | 50:21 | 29 | 59 |
| 2 |
|
FC Sellier & Bellot Vlašim | 25 | 14 | 3 | 8 | 51:33 | 18 | 45 |
| 3 |
|
AC Sparta Praha B | 25 | 13 | 4 | 8 | 44:29 | 15 | 43 |
| 4 |
|
SK Líšeň | 25 | 11 | 8 | 6 | 39:28 | 11 | 41 |
| 5 |
|
SFC Opava | 25 | 11 | 8 | 6 | 37:26 | 11 | 41 |
| 6 |
|
FK Varnsdorf | 25 | 11 | 5 | 9 | 40:31 | 9 | 38 |
| 7 |
|
FK Dukla Praha | 25 | 10 | 5 | 10 | 34:34 | 0 | 35 |
| 8 |
|
Vysočina Jihlava | 25 | 9 | 7 | 9 | 21:27 | -6 | 34 |
| 9 |
|
1. SK Prostějov | 25 | 10 | 3 | 12 | 26:39 | -13 | 33 |
| 10 |
|
1. FK Příbram | 25 | 8 | 7 | 10 | 32:40 | -8 | 31 |
| 11 |
|
FK MAS Táborsko | 25 | 7 | 9 | 9 | 26:30 | -4 | 30 |
| 12 |
|
MFK Vyškov | 25 | 8 | 5 | 12 | 39:40 | -1 | 29 |
| 13 |
|
MFK Chrudim | 25 | 7 | 8 | 10 | 24:32 | -8 | 29 |
| 14 |
|
FK Fotbal Třinec | 25 | 7 | 6 | 12 | 28:42 | -14 | 27 |
| 15 |
|
FK Ústí nad Labem | 25 | 5 | 9 | 11 | 22:38 | -16 | 24 |
| 16 |
|
Viktoria Žižkov | 25 | 2 | 7 | 16 | 20:43 | -23 | 13 |
| Aufstieg | |
| Playoffs | |
| Abstieg |
